Anti-IBSP Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB

A03183

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of IBSP using anti-IBSP antibody (A03183). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-IBSP antigen affinity purified polyclonal antibody (A03183) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for IBSP at approximately 55 kDa. The expected band size for IBSP is at 35 kDa.
all(1)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-IBSP Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Bone sialoprotein 2; BNSP; BSP; BSP-II; SP-II; Integrin-binding sialoprotein; IBSP

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

IBSP

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human IBSP, which shares 88.9% and 87% amino acid (aa) sequence identity with mouse and rat IBSP, respectively.

计算分子量

35 kDa

实际分子量

45-60 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

IBSP (integrin-binding sialoprotein) is also known as BSP. The protein encoded by this gene is a major structural protein of the bone matrix. Bone sialoprotein is an acidic glycoprotein of approximately 70 kD that undergoes extensive posttranslational modifications. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. The BSP gene is mapped to 4q22.1.

Uniprot ID

P21815

RRID

AB_3081619

文献引用格式

IBSP Antibody (Boster Biological Technology, Wuhan, China. Catalog#A03183)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

实验方案>

实验步骤

引用文献(0)>

清除