Anti-Osteoadherin/OMD Antibody

筛选器： All WB FCM ELISA

• 

  Western blot analysis of anti-OMD antibody (A05351-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: mouse heart tissue lysates,
  Lane 2: mouse bone tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OMD antigen affinity purified polyclonal antibody (A05351-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for OMD at approximately 60 kDa. The expected band size for OMD is at 49 kDa.

• 

  Flow Cytometry analysis of U2OS cells using anti-OMD antibody (A05351-1).
  Overlay histogram showing U2OS cells stained with A05351-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OMD Antibody (A05351-1, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

• 

  Western blot analysis of anti-OMD antibody (A05351-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: mouse heart tissue lysates,
  Lane 2: mouse bone tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OMD antigen affinity purified polyclonal antibody (A05351-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for OMD at approximately 60 kDa. The expected band size for OMD is at 49 kDa.

• 

  Flow Cytometry analysis of U2OS cells using anti-OMD antibody (A05351-1).
  Overlay histogram showing U2OS cells stained with A05351-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-OMD Antibody (A05351-1, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

•