Anti-FOX2/RBM9/RBFOX2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC IF FCM ELISA

A05389-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-FOX2/RBM9/RBFOX2 antibody (A05389-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U2OS whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human U87 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-FOX2/RBM9/RBFOX2 antigen affinity purified polyclonal antibody (A05389-2) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for FOX2/RBM9/RBFOX2 at approximately 60 kDa. The expected band size for FOX2/RBM9/RBFOX2 is at 41 kDa.
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IHC analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2).
FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2).
FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2).
FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2).
FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of human cortical adenomas tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IHC analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2).
FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
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IF analysis of FOX2/RBM9/RBFOX2 using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2).
FOX2/RBM9/RBFOX2 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-FOX2/RBM9/RBFOX2 Antibody (A05389-2) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody.
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Flow Cytometry analysis of SH-SY5Y cells using anti-FOX2/RBM9/RBFOX2 antibody (A05389-2).
Overlay histogram showing SH-SY5Y cells stained with A05389-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOX2/RBM9/RBFOX2 Antibody (A05389-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-FOX2/RBM9/RBFOX2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Fox 1 homolog B; Fox 2; FOX2; fxh; HNRBP2; HRNBP2; RBFOX2; RBM9; RNA binding motif protein 9; RNA binding protein 9; RTA

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IF, FCM, ELISA

基因名称

RBFOX2

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human FOX2/RBM9/RBFOX2 recombinant protein (Position: M1-Y256).

计算分子量

41 kDa

实际分子量

41,60 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

RNA binding motif protein 9 (RBM9), also known as Rbfox2, is a protein which in humans is encoded by the RBM9 gene. It is mapped to 22q12.3. This gene is one of several human genes similar to the C. elegans gene Fox-1. This gene encodes an RNA binding protein that is thought to be a key regulator of alternative exon splicing in the nervous system and other cell types. The protein binds to a conserved UGCAUG element found downstream of many alternatively spliced exons and promotes inclusion of the alternative exon in mature transcripts. The protein also interacts with the estrogen receptor 1 transcription factor and regulates estrogen receptor 1 transcriptional activity. Multiple transcript variants encoding different isoforms have been found for this gene.

Uniprot ID

O43251

文献引用格式

FOX2/RBM9/RBFOX2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A05389-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

RNA binding fox-1 homolog 2

蛋白名全称

RNA binding protein fox-1 homolog 2

实验方案>

实验步骤

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