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Anti-HAPLN1 Antibody

Rabbit polyclonal antibody

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筛选器: All WB IHC ELISA

A05980-3

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of HAPLN1 using anti-HAPLN1 antibody (A05980-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: human Caco-2 whole cell lysates,
    Lane 2: human U2OS whole cell lysates,
    Lane 3: human PC-3 whole cell lysates,
    Lane 4: rat brain tissue lysates,
    Lane 5: mouse brain tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HAPLN1 antigen affinity purified polyclonal antibody (A05980-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HAPLN1 at approximately 40,48 kDa. The expected band size for HAPLN1 is at 40,48 kDa.

    all(3)
  • IHC analysis of HAPLN1 using anti-HAPLN1 antibody (A05980-3) .
    HAPLN1 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-HAPLN1 Antibody (A05980-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.

    all(3)

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产品名称
Anti-HAPLN1 Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
Cartilage link protein; Cartilage linking protein 1; CRTL1; HAPLN1; Proteoglycan link protein
产品类型
Polyclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ELISA
基因名称
HAPLN1
宿主
Rabbit
抗体亚型
IgG
免疫原
E.coli-derived human HAPLN1 recombinant protein (Position: D16-N354). Human HAPLN1 shares 96.5% amino acid (aa) sequence identity with both mouse and rat HAPLN1.
计算分子量
40,48 kDa
实际分子量
40,48 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Predicted to enable hyaluronic acid binding activity. Predicted to be an extracellular matrix structural constituent conferring compression resistance. Predicted to be involved in nervous system development; positive regulation of neuroblast proliferation; and skeletal system development. Located in collagen-containing extracellular matrix.
Uniprot ID
P10915  
Gene ID
1404  
文献引用格式
HAPLN1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A05980-3)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
hyaluronan and proteoglycan link protein 1
蛋白名全称
Hyaluronan and proteoglycan link protein 1
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
Enzyme linked immunosorbent assay (ELISA):1:100-1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-HAPLN1 Antibody

    筛选器: All WB IHC ELISA

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