Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Western blot analysis of HAPLN1 using anti-HAPLN1 antibody (A05980-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human U2OS whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HAPLN1 antigen affinity purified polyclonal antibody (A05980-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HAPLN1 at approximately 40,48 kDa. The expected band size for HAPLN1 is at 40,48 kDa.
IHC analysis of HAPLN1 using anti-HAPLN1 antibody (A05980-3) .
HAPLN1 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-HAPLN1 Antibody (A05980-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of HAPLN1 using anti-HAPLN1 antibody (A05980-3). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human U2OS whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HAPLN1 antigen affinity purified polyclonal antibody (A05980-3) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HAPLN1 at approximately 40,48 kDa. The expected band size for HAPLN1 is at 40,48 kDa.
IHC analysis of HAPLN1 using anti-HAPLN1 antibody (A05980-3) .
HAPLN1 was detected in a paraffin-embedded section of human placenta tissue. The tissue section was incubated with rabbit anti-HAPLN1 Antibody (A05980-3) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.