Western blot analysis of NDUFB11 using anti-NDUFB11 antibody (A08638-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: rat skeletal muscle tissue lysates,
Lane 6: rat heart tissue lysates,
Lane 7: mouse skeletal muscle tissue lysates,
Lane 8: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NDUFB11 antigA03957-Aen affinity purified polyclonal antibody (A08638-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NDUFB11 at approximately 18 kDa. The expected band size for NDUFB11 is at 18 kDa.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| ImmunoPrecipitation (IP): | 1:50 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of NDUFB11 using anti-NDUFB11 antibody (A08638-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: rat skeletal muscle tissue lysates,
Lane 6: rat heart tissue lysates,
Lane 7: mouse skeletal muscle tissue lysates,
Lane 8: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NDUFB11 antigA03957-Aen affinity purified polyclonal antibody (A08638-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NDUFB11 at approximately 18 kDa. The expected band size for NDUFB11 is at 18 kDa.
Western blot analysis of NDUFB11 using anti-NDUFB11 antibody (A08638-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: rat skeletal muscle tissue lysates,
Lane 6: rat heart tissue lysates,
Lane 7: mouse skeletal muscle tissue lysates,
Lane 8: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NDUFB11 antigA03957-Aen affinity purified polyclonal antibody (A08638-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for NDUFB11 at approximately 18 kDa. The expected band size for NDUFB11 is at 18 kDa.
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