Anti-GRK2 Antibody

筛选器： All WB ICC/IF FCM

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  Western blot analysis of GRK2 using anti-GRK2 antibody (A32388-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Human THP-1 whole cell lysates,
  Lane 2: Monkey COS-7 whole cell lysates,
  Lane 3: Human Raji whole cell lysates,
  Lane 4: Rat lung tissue lysates,
  Lane 5: Mouse NIH/3T3 whole cell lysates,
  Lane 6: Mouse RAW264.7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GRK2 antigen affinity purified polyclonal antibody (A32388-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GRK2 at approximately 80 kDa. The expected band size for GRK2 is at 80 kDa.

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  IF analysis of GRK2 using anti-GRK2 antibody (A32388-1).
  GRK2 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-GRK2 Antibody (A32388-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Flow Cytometry analysis of Hepa1-6 cells using anti-GRK2 antibody (A32388-1).
  Overlay histogram showing Hepa1-6 cells stained with A32388-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRK2 Antibody (A32388-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Flow Cytometry analysis of RH-35 cells using anti-GRK2 antibody (A32388-1).
  Overlay histogram showing RH-35 cells stained with A32388-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRK2 Antibody (A32388-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Flow Cytometry analysis of U937 cells using anti-GRK2 antibody (A32388-1).
  Overlay histogram showing U937 cells stained with A32388-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRK2 Antibody (A32388-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of GRK2 using anti-GRK2 antibody (A32388-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Human THP-1 whole cell lysates,
  Lane 2: Monkey COS-7 whole cell lysates,
  Lane 3: Human Raji whole cell lysates,
  Lane 4: Rat lung tissue lysates,
  Lane 5: Mouse NIH/3T3 whole cell lysates,
  Lane 6: Mouse RAW264.7 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GRK2 antigen affinity purified polyclonal antibody (A32388-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GRK2 at approximately 80 kDa. The expected band size for GRK2 is at 80 kDa.

• 

  IF analysis of GRK2 using anti-GRK2 antibody (A32388-1).
  GRK2 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-GRK2 Antibody (A32388-1) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

• 

  Flow Cytometry analysis of Hepa1-6 cells using anti-GRK2 antibody (A32388-1).
  Overlay histogram showing Hepa1-6 cells stained with A32388-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRK2 Antibody (A32388-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Flow Cytometry analysis of RH-35 cells using anti-GRK2 antibody (A32388-1).
  Overlay histogram showing RH-35 cells stained with A32388-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRK2 Antibody (A32388-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Flow Cytometry analysis of U937 cells using anti-GRK2 antibody (A32388-1).
  Overlay histogram showing U937 cells stained with A32388-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GRK2 Antibody (A32388-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.