Western blot analysis of AGTR1 using anti-AGTR1 antibody (BA0582). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AGTR1 antigen affinity purified polyclonal antibody (BA0582) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AGTR1 at approximately 50 kDa. The expected band size for AGTR1 is at 41 kDa.
IHC analysis of AGTR1 using anti-AGTR1 antibody (BA0582).
AGTR1 was detected in a paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-AGTR1 Antibody (BA0582) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of AGTR1 using anti-AGTR1 antibody (BA0582). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AGTR1 antigen affinity purified polyclonal antibody (BA0582) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AGTR1 at approximately 50 kDa. The expected band size for AGTR1 is at 41 kDa.
IHC analysis of AGTR1 using anti-AGTR1 antibody (BA0582).
AGTR1 was detected in a paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-AGTR1 Antibody (BA0582) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of AGTR1 using anti-AGTR1 antibody (BA0582). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AGTR1 antigen affinity purified polyclonal antibody (BA0582) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AGTR1 at approximately 50 kDa. The expected band size for AGTR1 is at 41 kDa.
IHC analysis of AGTR1 using anti-AGTR1 antibody (BA0582).
AGTR1 was detected in a paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-AGTR1 Antibody (BA0582) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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