Anti-Annexin A1/ANXA1 Antibody

Rabbit polyclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC IP

BA0640

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of Annexin A1/ANXA1 using anti-Annexin A1/ANXA1 antibody (BA0640). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse C2C12 whole cell lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Annexin A1/ANXA1 antigen affinity purified polyclonal antibody (BA0640) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Annexin A1/ANXA1 at approximately 37 kDa. The expected band size for Annexin A1/ANXA1 is at 39 kDa.
all(4)
IHC analysis of Annexin A1/ANXA1 using anti-Annexin A1/ANXA1 antibody (BA0640) .
Annexin A1/ANXA1 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-Annexin A1/ANXA1 Antibody (BA0640) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of Annexin A1/ANXA1 using anti-Annexin A1/ANXA1 antibody (BA0640) .
Annexin A1/ANXA1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-Annexin A1/ANXA1 Antibody (BA0640) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IP analysis of Annexin A1/ANXA1 using anti-Annexin A1/ANXA1 antibody (BA0640) in A431 whole cell lysate.
Western blot analysis of Annexin A1/ANXA1 using anti- Annexin A1/ANXA1 antibody (BA0640).
Lane 1: A431 whole cell lysates(30ug),
Lane 2: Rabbit control IgG instead of anti- Annexin A1/ANXA1 antibody in A431 whole cell lysate,
Lane 3: anti- Annexin A1/ANXA1 antibody (2μg) + A431 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- Annexin A1/ANXA1 antigen affinity purified polyclonal antibody (BA0640) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Annexin A1/ANXA1 at approximately 37 kDa. The expected band size for Annexin A1/ANXA1 is at 39 kDa.
all(4)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Annexin A1/ANXA1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Annexin 1; annexin A1; Annexin I; ANX1; ANXA1; Calpactin 2; Calpactin II; Chromobindin 9; Lipocortin I; LPC1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IP

基因名称

ANXA1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human Annexin A1.

计算分子量

39 kDa

实际分子量

37 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Annexin I, also known as lipocortin I(Lipo1), belongs to the family of annexins. These proteins are though to control the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes. In two lipocortins(I and II) a short amino-terminal sequence distinct from the core structure has potential regulatory functions which are dependent on its phosphorylation state. The gene in the mouse encodes a protein of 346 amino acid residues. Mouse Lipo1 gene spans about 17 kb and is divided into 13 exons. Annexin I gene, mapped to 9q11-q22, is located on mouse chromosome 19. Annexin I acts through the formyl peptide receptor on human neutrophils. Peptides derived from the unique N-terminal domain of annexin I serve as FPR ligands and trigger different signaling pathways in a dose-dependent manner.

Uniprot ID

P04083

文献引用格式

Annexin A1/ANXA1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0640)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

annexin A1

蛋白名全称

Annexin A1

实验方案>

实验步骤

引用文献(0)>

清除