Western blot analysis of Annexin V/ANXA5 using anti-Annexin V/ANXA5 antibody (BA0644). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat brain tissue lysates,
Lane 2: Rat skeletal muscle tissue lysates,
Lane 3: Rat ovary tissue lysates,
Lane 4: Rat lung tissue lysates,
Lane 5: MCF-7 whole cell lysates,
Lane 6: SMMC whole cell lysates,
Lane 7: A549 whole cell lysates,
Lane 8: JURKAT whole cell lysates,
Lane 9: SGC whole cell lysates,
Lane 10: HT1080 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Annexin V/ANXA5 antigen affinity purified polyclonal antibody (BA0644) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Annexin V/ANXA5 at approximately 36 kDa. The expected band size for Annexin V/ANXA5 is at 36 kDa.
IHC analysis of Annexin V/ANXA5 using anti-Annexin V/ANXA5 antibody (BA0644).
Annexin V/ANXA5 was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Annexin V/ANXA5 Antibody (BA0644) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
ICC analysis of Annexin V/ANXA5 using anti- Annexin V/ANXA5 antibody (BA0644).
Annexin V/ANXA5 was detected in an immunocytochemical section of HCT116 cells. The section was incubated with rabbit anti-Annexin V/ANXA5 Antibody (BA0644) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
ICC analysis of Annexin V/ANXA5 using anti- Annexin V/ANXA5 antibody (BA0644).
Annexin V/ANXA5 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-Annexin V/ANXA5 Antibody (BA0644) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of Annexin V/ANXA5 using anti-Annexin V/ANXA5 antibody (BA0644). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat brain tissue lysates,
Lane 2: Rat skeletal muscle tissue lysates,
Lane 3: Rat ovary tissue lysates,
Lane 4: Rat lung tissue lysates,
Lane 5: MCF-7 whole cell lysates,
Lane 6: SMMC whole cell lysates,
Lane 7: A549 whole cell lysates,
Lane 8: JURKAT whole cell lysates,
Lane 9: SGC whole cell lysates,
Lane 10: HT1080 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Annexin V/ANXA5 antigen affinity purified polyclonal antibody (BA0644) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Annexin V/ANXA5 at approximately 36 kDa. The expected band size for Annexin V/ANXA5 is at 36 kDa.
IHC analysis of Annexin V/ANXA5 using anti-Annexin V/ANXA5 antibody (BA0644).
Annexin V/ANXA5 was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Annexin V/ANXA5 Antibody (BA0644) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
ICC analysis of Annexin V/ANXA5 using anti- Annexin V/ANXA5 antibody (BA0644).
Annexin V/ANXA5 was detected in an immunocytochemical section of HCT116 cells. The section was incubated with rabbit anti-Annexin V/ANXA5 Antibody (BA0644) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
ICC analysis of Annexin V/ANXA5 using anti- Annexin V/ANXA5 antibody (BA0644).
Annexin V/ANXA5 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-Annexin V/ANXA5 Antibody (BA0644) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of Annexin V/ANXA5 using anti-Annexin V/ANXA5 antibody (BA0644). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat brain tissue lysates,
Lane 2: Rat skeletal muscle tissue lysates,
Lane 3: Rat ovary tissue lysates,
Lane 4: Rat lung tissue lysates,
Lane 5: MCF-7 whole cell lysates,
Lane 6: SMMC whole cell lysates,
Lane 7: A549 whole cell lysates,
Lane 8: JURKAT whole cell lysates,
Lane 9: SGC whole cell lysates,
Lane 10: HT1080 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Annexin V/ANXA5 antigen affinity purified polyclonal antibody (BA0644) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Annexin V/ANXA5 at approximately 36 kDa. The expected band size for Annexin V/ANXA5 is at 36 kDa.
IHC analysis of Annexin V/ANXA5 using anti-Annexin V/ANXA5 antibody (BA0644).
Annexin V/ANXA5 was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Annexin V/ANXA5 Antibody (BA0644) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
ICC analysis of Annexin V/ANXA5 using anti- Annexin V/ANXA5 antibody (BA0644).
Annexin V/ANXA5 was detected in an immunocytochemical section of HCT116 cells. The section was incubated with rabbit anti-Annexin V/ANXA5 Antibody (BA0644) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
ICC analysis of Annexin V/ANXA5 using anti- Annexin V/ANXA5 antibody (BA0644).
Annexin V/ANXA5 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-Annexin V/ANXA5 Antibody (BA0644) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
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