Anti-AQP2 Antibody

Rabbit polyclonal antibody

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筛选器： All IHC WB

BA0649

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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IHC analysis of AQP2 using anti-AQP2 antibody (BA0649).
AQP2 was detected in a paraffin-embedded section of rat kidney tissue. The tissue section was incubated with rabbit anti-AQP2 Antibody (BA0649) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(3)
Western blot analysis of AQP2 using anti-AQP2 antibody (BA0649). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates,
Lane 2: SW620 whole cell lysates,
Lane 3: HT1080 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AQP2 antigen affinity purified polyclonal antibody (BA0649) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AQP2 at approximately 26-37 kDa. The expected band size for AQP2 is at 29 kDa.
all(3)
IHC analysis of AQP2 using anti-AQP2 antibody (BA0649).
AQP2 was detected in frozen section of rat kidney tissue. The tissue section was incubated with rabbit anti-AQP2 Antibody (BA0649) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(3)

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产品简介>

产品名称

Anti-AQP2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ADH water channel; AQP 2; AQP CD; AQP2; Aquaporin 2; aquaporin 2 (collecting duct); Aquaporin CD; WCH CD

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

IHC, WB

基因名称

AQP2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 2, identical to the related rat and mouse sequences.

计算分子量

29 kDa

实际分子量

26-37 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

AQP2(Aquaporin 2) also called AQUAPORIN-CD, is found in the apical cell membranes of the kidney's collecting duct principal cells and in intracellular vesicles located throughout the cell. The AQP2 gene is mapped to chromosome 12q13, very close to the site of major intrinsic protein by situ hybridization. The investigators suggested that a defect in the AQP2 gene is the basis of the autosomal form of nephrogenic diabetes insipidus. The functional expression and the limited localization suggested that AQP2 is the vasopressin-regulated water channel. Using rat kidney slices and porcine kidney cells stably expressing rat Aqp2, AQP2 trafficking can be stimulated by cAMP-independent pathways that utilize nitric oxide(NO). The NO donors sodium nitroprusside(SNP) and NONOate and the NO synthase substrate L-arginine mimicked the effect of vasopressin(VP), stimulating relocation of Aqp2 from cytoplasmic vesicles to the apical plasma membrane. SNP increased intracellular cGMP rather than cAMP, and exogenous cGMP stimulated AQP2 membrane insertion. Atrial natriuretic factor, which signals via cGMP, also stimulated AQP2 translocation. AQP2 expression in kidney connecting tubules is sufficient for survival and that AQP2 expression in collecting ducts is required to regulate body water balance. The S256L substitution in the cytoplasmic tail of the Aqp2 protein prevented phosphorylation at S256 and the subsequent accumulation of Aqp2 on the apical membrane of the collecting duct principal cells.

Uniprot ID

P41181

文献引用格式

AQP2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA0649)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

aquaporin 2(collecting duct)

蛋白名全称

Aquaporin-2(AQP-2)

实验方案>

实验步骤

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