DyLight 647 Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)

DyLight 647-山羊抗兔IgG

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筛选器： All WB IF ICC/IF FCM

BA1150

100μl ￥240 1ml ￥1920
货期：现货

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Western blot analysis of APEX1 using anti-APEX1 antibody (PB9128). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human Raji whole cell lysates,
Lane 4: human A431 whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: human MCF-7 whole cell lysates,
Lane 7: human RT4 whole cell lysates,
Lane 8: human U87 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-APEX1 antigen affinity purified polyclonal antibody (PB9128) at a dilution of 1:2000 and probed with a DyLight 647 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1150). A specific band was detected for APEX1 at approximately 36 kDa. The expected band size for APEX1 is at 36 kDa.
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IF analysis of COX5B using anti-COX5B antibody (A06090-2).
COX5B was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-COX5B Antibody (A06090-2) at a dilution of 1:100. DyLight 647 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) secondary antibody (Catalog # BA1150) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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产品简介>

产品名称

DyLight 647 Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)

规格/价格

100μl/240 1ml/1920

浓度

1 mg/ml

抗体亚型

IgG

免疫原

Rabbit IgG (whole molecular)

宿主

Goat

应用范围

WB, IF, ICC/IF, FCM

纯化方式

The antibody was purified from antisera by immunoaffinity chromatography.

纯度

>99%

产品形态

Concentrated, Liquid

成分

1 mg/ml of DyLight 647 conjugated specific antibody; 0.01M PBS, 5 mg/ml BSA, 50% glycerol.

特异性

This DyLight 647 conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with human/bovine/mouse IgG.

激发/发射光谱

Amax=652nm; Emax=664nm

偶联物

DyLight 647

应用注释

Please centrifuge before use diluted by neutral PBS or TBS. The diluted solution should be used on the same day. Any unused solution should be disposed of.

文献引用格式

DyLight 647 Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (Boster Biological Technology, Wuhan, China. Catalog#BA1150)

建议工作浓度

WB: 1:2000-10000;
IF: 1:100—500;
Optimal working dilutions must be determined by end users.

保存条件

At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protected from light.

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