DyLight 647 Conjugated AffiniPure Goat Anti-Mouse IgG (H+L)

DyLight 647-山羊抗小鼠IgG

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筛选器： All WB IF ICC/IF FCM

BA1151

100μl ￥240 1ml ￥1920
货期：现货

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Western blot analysis of anti-Importin Beta/KPNB1 antibody (M01851-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat NRK whole cell lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Importin Beta/KPNB1 antigen affinity purified monoclonal antibody (M01851-2) at a dilution of 1:1000 and probed with a DyLight 647 Conjugated AffiniPure Goat Anti-mouse IgG (H+L) secondary antibody (Catalog # BA1151). A specific band was detected for Importin Beta/KPNB1 at approximately 97 kDa. The expected band size for Importin Beta/KPNB1 is at 97 kDa.
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IF analysis of DDX1 using anti-DDX1 antibody (M03727).
DDX1 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with mouse anti-DDX1 Antibody (M03727) at a dilution of 1:100. DyLight 647 Conjugated AffiniPure Goat Anti-mouse IgG (H+L) secondary antibody (Catalog # BA1151) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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IF analysis of NEFH using anti-NEFH antibody (BM0100).
NEFH was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with mouse anti-NEFH Antibody (BM0100) at a dilution of 1:100. DyLight 647 Conjugated AffiniPure Goat Anti-mouse IgG (H+L) secondary antibody (Catalog # BA1151) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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产品简介>

产品名称

DyLight 647 Conjugated AffiniPure Goat Anti-Mouse IgG (H+L)

规格/价格

100μl/240 1ml/1920

浓度

1 mg/ml

抗体亚型

IgG

免疫原

Mouse IgG (whole molecular)

宿主

Goat

应用范围

WB, IF, ICC/IF, FCM

纯化方式

The antibody was purified from antisera by immunoaffinity chromatography.

纯度

>99%

产品形态

Concentrated, Liquid

成分

1 mg/ml of DyLight 647 conjugated specific antibody; 0.01M PBS, 5 mg/ml BSA, 50% glycerol.

特异性

This DyLight 647 conjugated antibody is specific for mouse IgG and shows no cross-reactivity with human/bovine/rabbit IgG.

激发/发射光谱

Amax=652nm; Emax=664nm

偶联物

DyLight 647

应用注释

Please centrifuge before use diluted by neutral PBS or TBS. The diluted solution should be used on the same day. Any unused solution should be disposed of.

文献引用格式

DyLight 647 Conjugated AffiniPure Goat Anti-Mouse IgG (H+L) (Boster Biological Technology, Wuhan, China. Catalog#BA1151)

建议工作浓度

WB: 1:2000-10000;
IF: 1:100—500;
Optimal working dilutions must be determined by end users.

保存条件

At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protected from light.

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