Anti-Lamin A/C Antibody

Rabbit Polyclonal Antibody

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筛选器： All WB

BA1227

100μl ￥2180 50μl ￥1280 150μl ￥2800
货期：现货

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Western blot analysis of Lamin A/C using anti-Lamin A/C antibody (BA1227). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Human placenta tissue lysates,
Lane 2: Human PC-3 whole cell lysates,
Lane 3: Human HELA whole cell lysates,
Lane 4: Human HepG2 whole cell lysates,
Lane 5: Human CACO-2 whole cell lysates,
Lane 6: rat spleen tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Lamin A/C antigen affinity purified polyclonal antibody (BA1227) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Lamin A/C at approximately 74 kDa. The expected band size for Lamin A/C is at 74 kDa.
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产品名称

Anti-Lamin A/C Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

70 kDa lamin; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; HGPS; IDC; lamin A; lamin A/C; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; Prelamin A/C; PRO1; progerin

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

LMNA

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin A/C, identical to the related rat and mouse sequences.

计算分子量

74 kDa

实际分子量

74 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Lamins are structural protein components of the nuclear lamina, a protein network underlying the inner nuclear membrane that determines nuclear shape and size. There are three types of lamins, A,B and C. The lamin A/C(LMNA) gene contains 12 exons. Alternative splicing within exon 10 gives rise to two different mRNAs that code for pre-lamin A and lamin C. Lamin A/C mapped to 1q21.2-q21.3 and mutations in this gene cause a variety of human diseases including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, and Hutchinson-Gilford progeria syndrome. Lamin A/C deficiency is thus associated with both defective nuclear mechanics and impaired mechanically activated gene transcription.

Uniprot ID

P02545

文献引用格式

Lamin A/C Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1227)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

lamin A/C

实验方案>

实验步骤

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