Western blot (WB): | 1:500-2000 |
Western blot analysis of Tenascin-C/TNC using anti-Tenascin-C/TNC antibody (BA1428-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat brain tissue lysates,
Lane 2: Rat liver tissue lysates,
Lane 3: Rat spleen tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Tenascin-C/TNC antigen affinity purified polyclonal antibody (BA1428-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Tenascin-C/TNC at approximately 241 kDa. The expected band size for Tenascin-C/TNC is at 232 kDa.
Western blot analysis of Tenascin-C/TNC using anti-Tenascin-C/TNC antibody (BA1428-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat brain tissue lysates,
Lane 2: Rat liver tissue lysates,
Lane 3: Rat spleen tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Tenascin-C/TNC antigen affinity purified polyclonal antibody (BA1428-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Tenascin-C/TNC at approximately 241 kDa. The expected band size for Tenascin-C/TNC is at 232 kDa.