Anti-SERCA2/ATP2A2 Antibody

Rabbit polyclonal antibody

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筛选器： All IHC WB

BA1744

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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IHC analysis of SERCA2/ATP2A2 using anti-SERCA2/ATP2A2 antibody (BA1744).
SERCA2/ATP2A2 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-SERCA2/ATP2A2 Antibody (BA1744) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(2)
Western blot analysis of SERCA2/ATP2A2 using anti-SERCA2/ATP2A2 antibody (BA1744). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Skeletal Muscle tissue lysates,
Lane 2: Rat Kidney tissue lysates,
Lane 3: PANC whole cell lysates,
Lane 4: SMMC whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SERCA2/ATP2A2 antigen affinity purified polyclonal antibody (BA1744) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for SERCA2/ATP2A2 at approximately 115 kDa. The expected band size for SERCA2/ATP2A2 is at 115 kDa.
all(2)

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产品简介>

产品名称

Anti-SERCA2/ATP2A2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ATP2A2; ATP2B; Calcium pump 2; DAR; DD; DKFZp686P0211; FLJ20293; FLJ38063; SERCA2; SERCA2; ATP2A2; SERCA2b; SR Ca(2+) ATPase 2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

IHC, WB

基因名称

ATP2A2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human SERCA2 ATPase, identical to the related rat and mouse sequences.

计算分子量

115 kDa

实际分子量

115 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

SERCA2(SARCOPLASMIC RETICULUM Ca(2+)-ATPase 2), also called ATP2A2, ATP2B, encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. They are closely related to the plasma membrane Ca(2+)-ATPases, or PMCAs. SERCA2 belongs to the large family of P-type cation pumps that couple ATP hydrolysis with cation transport across membranes. The SERCA2 gene is mapped on 12q24.11. SERCA2 was expressed in all specimens, with pronounced expression in the subnuclear aspect of basal epidermal keratinocytes. There was variable suprabasal expression.SERCA2 expression was also observed in the infundibulum and outer root sheath of hair follicles; germinative and mature cells of sebaceous glands; secretory coil and duct of eccrine glands; apocrine gland cells; and arrector pili muscle. In Darier disease skin, strong SERCA2 positivity was detected in the basal, suprabasal, and acantholytic lesional cells. Perilesional Darier disease skin was comparable to normal skin.

Uniprot ID

P16615

文献引用格式

SERCA2/ATP2A2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA1744)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

ATPase, Ca++ transporting, cardiac muscle, slow twitch 2

蛋白名全称

Sarcoplasmic/endoplasmic reticulum calcium ATPase 2(SERCA2/SR Ca(2+)-ATPase 2)

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实验步骤

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