Anti-PGK1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA3015-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of PGK1 using anti-PGK1 antibody (BA3015-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PGK1 antigen affinity purified polyclonal antibody (BA3015-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PGK1 at approximately 45 kDa. The expected band size for PGK1 is at 45 kDa.
all(2)
IHC analysis of PGK1 using anti-PGK1 antibody (BA3015-2).
PGK1 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-PGK1 Antibody (BA3015-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品简介>

产品名称

Anti-PGK1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

MIG10; OK/SW cl.110; PGK1; PGKA; phosphoglycerate kinase 1; Primer recognition protein 2; PRP 2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

PGK1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human PGK1, identical to the related rat and mouse sequences.

计算分子量

45 kDa

实际分子量

45 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

PGK1 (Phosphoglycerate Kinase 1), also known as PGKA, is an enzyme that in humans is encoded by the PGK1 gene. The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. And the encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome.

Uniprot ID

P00558

文献引用格式

PGK1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3015-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

phosphoglycerate kinase 1

蛋白名全称

Phosphoglycerate kinase 1

实验方案>

实验步骤

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