Western blot (WB): | 1:500-2000 |
Western blot analysis of ITGA7 using anti-ITGA7 antibody (BA3357-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: 293T whole cell lysates,
Lane 2: A431 whole cell lysates,
Lane 3: HELA whole cell lysates,
Lane 4: JURKAT whole cell lysates,
Lane 5: RAJI whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ITGA7 antigen affinity purified polyclonal antibody (BA3357-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ITGA7 at approximately 130 kDa. The expected band size for ITGA7 is at 129 kDa.
Western blot analysis of ITGA7 using anti-ITGA7 antibody (BA3357-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: 293T whole cell lysates,
Lane 2: A431 whole cell lysates,
Lane 3: HELA whole cell lysates,
Lane 4: JURKAT whole cell lysates,
Lane 5: RAJI whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ITGA7 antigen affinity purified polyclonal antibody (BA3357-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ITGA7 at approximately 130 kDa. The expected band size for ITGA7 is at 129 kDa.