Anti-CUL4B Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA3374-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CUL4B using anti-CUL4B antibody (BA3374-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: rat thymus tissue lysates,
Lane 4: rat kidney tissue lysates,
Lane 5: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CUL4B antigen affinity purified polyclonal antibody (BA3374-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CUL4B at approximately 104 kDa. The expected band size for CUL4B is at 104 kDa.
all(2)
IHC analysis of CUL4B using anti-CUL4B antibody (BA3374-2).
CUL4B was detected in a paraffin-embedded section of zebrafish body tissue. The tissue section was incubated with rabbit anti-CUL4B Antibody (BA3374-2) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(2)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-CUL4B Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CUL 4B; CUL4B; cullin 4B; KIAA0695; MRXHF2; MRXSC; SFM2

产品类型

Polyclonal

检验物种

human, mouse, rat, zebrafish

应用范围

WB, IHC

基因名称

CUL4B

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human Cullin 4B, identical to the related rat and mouse sequences.

计算分子量

104 kDa

实际分子量

104 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Cullin 4B/CUL4B encodes a scaffold protein that organizes a cullin-RING(really interesting new gene) ubiquitin ligase(E3) complex in ubiquitylation. The CUL4 gene encodes a protein of 913 amino acids. The cullin domain is located between amino acid residues 217 and 815 and is characterized a by C-terminal globular domain(cullin homology domain) and a series of N-terminal repeats(cullin repeats). Ohtake et al.(2007) characterize a fat-soluble ligand-dependent ubiquitin ligase complex in human cell lines, in which dioxin receptor(AhR) is integrated as a component of a novel cullin 4B ubiquitin ligase complex, CUL4B(AhR). Complex assembly and ubiquitin ligase activity of CUL4B(AhR) in vitro and in vivo are dependent on the AhR ligand. In the CUL4B(AhR) complex, ligand-activated AhR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Their findings uncover a function for AhR as an atypical component of the ubiquitin ligase complex and demonstrate a non-genomic signalling pathway in which fat-soluble ligands regulate target-protein-selective degradation through a ubiquitin ligase complex.

Uniprot ID

Q13620

文献引用格式

CUL4B Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3374-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

cullin 4B

蛋白名全称

Cullin-4B(CUL-4B)

实验方案>

实验步骤

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