Western blot (WB): | 1:500-2000 |
Western blot analysis of Kv1.1/KCNA1 using anti-Kv1.1/KCNA1 antibody (BA3431-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat brain tissue lysates,
Lane 2: Rat testis tissue lysates,
Lane 3: Rat cardiac muscle tissue lysates,
Lane 4: HELA whole cell lysates,
Lane 5: U87 whole cell lysates,
Lane 6: SHG whole cell lysates,
Lane 7: NEURO whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Kv1.1/KCNA1 antigen affinity purified polyclonal antibody (BA3431-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Kv1.1/KCNA1 at approximately 56,90 kDa. The expected band size for Kv1.1/KCNA1 is at 56 kDa.
Western blot analysis of Kv1.1/KCNA1 using anti-Kv1.1/KCNA1 antibody (BA3431-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat brain tissue lysates,
Lane 2: Rat testis tissue lysates,
Lane 3: Rat cardiac muscle tissue lysates,
Lane 4: HELA whole cell lysates,
Lane 5: U87 whole cell lysates,
Lane 6: SHG whole cell lysates,
Lane 7: NEURO whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Kv1.1/KCNA1 antigen affinity purified polyclonal antibody (BA3431-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Kv1.1/KCNA1 at approximately 56,90 kDa. The expected band size for Kv1.1/KCNA1 is at 56 kDa.