Western blot analysis of CTBP2 using anti-CTBP2 antibody (BA3643). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CTBP2 antigen affinity purified polyclonal antibody (BA3643) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CTBP2 at approximately 49 kDa. The expected band size for CTBP2 is at 49 kDa.
IHC analysis of CTBP2 using anti-CTBP2 antibody (BA3643).
CTBP2 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CTBP2 using anti-CTBP2 antibody (BA3643).
CTBP2 was detected in a paraffin-embedded section of rat intestine tissue. The tissue section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CTBP2 using anti-CTBP2 antibody (BA3643).
CTBP2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CTBP2 using anti-CTBP2 antibody (BA3643).
CTBP2 was detected in a paraffin-embedded section of mouse intestine tissue. The tissue section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CTBP2 using anti-CTBP2 antibody (BA3643).
CTBP2 was detected in frozen section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CTBP2 using anti-CTBP2 antibody (BA3643).
CTBP2 was detected in frozen section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
ICC analysis of CTBP2 using anti- CTBP2 antibody (BA3643).
CTBP2 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of CTBP2 using anti-CTBP2 antibody (BA3643) and anti-Beta Tubulin antibody (M05613-4).
CTBP2 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.
Flow Cytometry analysis of HEL cells using anti-CTBP2 antibody (BA3643).
Overlay histogram showing HEL cells stained with BA3643 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTBP2 Antibody (BA3643) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Western blot analysis of CTBP2 using anti-CTBP2 antibody (BA3643). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CTBP2 antigen affinity purified polyclonal antibody (BA3643) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CTBP2 at approximately 49 kDa. The expected band size for CTBP2 is at 49 kDa.
IHC analysis of CTBP2 using anti-CTBP2 antibody (BA3643).
CTBP2 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CTBP2 using anti-CTBP2 antibody (BA3643).
CTBP2 was detected in a paraffin-embedded section of rat intestine tissue. The tissue section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CTBP2 using anti-CTBP2 antibody (BA3643).
CTBP2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CTBP2 using anti-CTBP2 antibody (BA3643).
CTBP2 was detected in a paraffin-embedded section of mouse intestine tissue. The tissue section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CTBP2 using anti-CTBP2 antibody (BA3643).
CTBP2 was detected in frozen section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CTBP2 using anti-CTBP2 antibody (BA3643).
CTBP2 was detected in frozen section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
ICC analysis of CTBP2 using anti- CTBP2 antibody (BA3643).
CTBP2 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of CTBP2 using anti-CTBP2 antibody (BA3643) and anti-Beta Tubulin antibody (M05613-4).
CTBP2 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.
Flow Cytometry analysis of HEL cells using anti-CTBP2 antibody (BA3643).
Overlay histogram showing HEL cells stained with BA3643 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTBP2 Antibody (BA3643) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Western blot analysis of CTBP2 using anti-CTBP2 antibody (BA3643). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CTBP2 antigen affinity purified polyclonal antibody (BA3643) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CTBP2 at approximately 49 kDa. The expected band size for CTBP2 is at 49 kDa.
IHC analysis of CTBP2 using anti-CTBP2 antibody (BA3643).
CTBP2 was detected in a paraffin-embedded section of human mammary cancer tissue. The tissue section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CTBP2 using anti-CTBP2 antibody (BA3643).
CTBP2 was detected in a paraffin-embedded section of rat intestine tissue. The tissue section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CTBP2 using anti-CTBP2 antibody (BA3643).
CTBP2 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CTBP2 using anti-CTBP2 antibody (BA3643).
CTBP2 was detected in a paraffin-embedded section of mouse intestine tissue. The tissue section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CTBP2 using anti-CTBP2 antibody (BA3643).
CTBP2 was detected in frozen section of rat intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IHC analysis of CTBP2 using anti-CTBP2 antibody (BA3643).
CTBP2 was detected in frozen section of mouse intestine tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
ICC analysis of CTBP2 using anti- CTBP2 antibody (BA3643).
CTBP2 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
IF analysis of CTBP2 using anti-CTBP2 antibody (BA3643) and anti-Beta Tubulin antibody (M05613-4).
CTBP2 was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-CTBP2 Antibody (BA3643) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) and Dylight488-conjugated Anti-mouse IgG Secondary Antibody (Green) (Catalog # BA1126) were used as secondary antibody.
Flow Cytometry analysis of HEL cells using anti-CTBP2 antibody (BA3643).
Overlay histogram showing HEL cells stained with BA3643 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTBP2 Antibody (BA3643) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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