Anti-TNFR1/TNFRSF1A Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

BA4891

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of TNFR1/TNFRSF1A using anti-TNFR1/TNFRSF1A antibody (BA4891). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SW579 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TNFR1/TNFRSF1A antigen affinity purified polyclonal antibody (BA4891) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TNFR1/TNFRSF1A at approximately 55-60 kDa. The expected band size for TNFR1/TNFRSF1A is at 50 kDa.
all(2)
IHC analysis of TNFR1/TNFRSF1A using anti-TNFR1/TNFRSF1A antibody (BA4891).
TNFR1/TNFRSF1A was detected in a paraffin-embedded section of human mammary tissue. The tissue section was incubated with rabbit anti-TNFR1/TNFRSF1A Antibody (BA4891) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品名称

Anti-TNFR1/TNFRSF1A Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

CD120a; FPF; p55; p55 R; p60; TBP1; TBPI; TNF R; TNF R I; TNF R1; TNF R55; TNF Receptor 1; TNF Receptor I; TNF RI; TNFAR; TNFR I; TNFR1; TNFR55; TNFR60; TNFRSF1A

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC

基因名称

TNFRSF1A

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human TNF Receptor I.

计算分子量

50 kDa

实际分子量

55-60 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Tumor necrosis factor receptor 1(TNFR1), a potent cytokine, elicits a broad spectrum of biologic responses which are mediated by binding to a cell surface receptor. Its gene is located on 12p13.2. The coding region and the 3-prime untranslated region of TNFR1 are distributed over 10 exons. There are 2 different proteins that serve as major receptors for TNF-alpha, one associated with myeloid cells and one associated with epithelial cells. Additionally, TNFR1 associates with the MADD protein through a death domain-death domain interaction. MADD provides a physical link between TNFR1 and the induction of mitogen-activated protein(MAP) kinase(e.g., ERK2) activation and arachidonic acid release. TNFR1-induced apoptosis involves 2 sequential signaling complexes. Complex I, the initial plasma membrane-bound complex, consists of TNFR1, the adaptor TRADD, the kinase RIP1, and TRAF2 and rapidly signals activation of NF-kappa-B. In a second step, TRADD and RIP1 associate with FADD and caspase-8, forming a cytoplasmic complex, complex II.

Uniprot ID

P19438

文献引用格式

TNFR1/TNFRSF1A Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA4891)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

tumor necrosis factor receptor superfamily, member 1A

蛋白名全称

Tumor necrosis factor receptor superfamily member 1A

实验方案>

实验步骤

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