欢迎访问博士德生物官网! 服务热线:027-67845390 |
您当前的位置: 首页 > 产品中心 > 一抗 > 多克隆抗体

Anti-MAOB Antibody

Rabbit polyclonal antibody

说明书 一键复制产品信息

筛选器: All WB IHC

BA4989

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of MAOB using anti-MAOB antibody (BA4989). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
    Lane 1: Mouse liver tissue lysates,
    Lane 2: Mouse lung tissue lysates,
    Lane 3: Rat kidney tissue lysates,
    Lane 4: Rat brain tissue lysates,
    Lane 5: Rat liver tissue lysates,
    Lane 6: Rat lung tissue lysates.
    After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MAOB antigen affinity purified polyclonal antibody (BA4989) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MAOB at approximately 59 kDa. The expected band size for MAOB is at 59 kDa.

    all(2)
  • IHC analysis of MAOB using anti-MAOB antibody (BA4989).
    MAOB was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MAOB Antibody (BA4989) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.

    all(2)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称
Anti-MAOB Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
MAO B; MAOB; Monoamine Oxidase B; Monoamine oxidase type B
产品类型
Polyclonal
检验物种
mouse, rat
应用范围
WB, IHC
基因名称
MAOB
宿主
Rabbit
抗体亚型
IgG
免疫原
A synthetic peptide corresponding to a sequence at the N-terminus of mouse MAOB, identical to the related rat sequence.
计算分子量
59 kDa
实际分子量
59 kDa
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied.
背景资料
MAOB(MONOAMINE OXIDASE B), also called MAO, BRAIN, AMINE OXIDASE(FLAVIN-CONTAINING) B, is a protein that in humans is encoded by the MAOB gene. MAOB is a member of the flavin monoamine oxidase family. And it is mapped on Xp11.3. MAOB catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine. Like MAOA, it also degrades dopamine. MAO-B is involved in the breakdown of dopamine, a neurotransmitter implicated in reinforcing and motivating behaviors as well as movement. MAO-B inhibition is, therefore, associated with enhanced activity of dopamine, as well as with decreased production of hydrogen peroxide, a source of reactive oxygen species.
Uniprot ID
Q8BW75  
文献引用格式
MAOB Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA4989)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
基因名全称
monoamine oxidase B
蛋白名全称
Amine oxidase[flavin-containing] B
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

实验方案>

实验步骤

引用文献(0)>

    相关产品>

    成功添加到购物车

    查看购物车 继续购物

    Anti-MAOB Antibody

    筛选器: All WB IHC

    亮暗模式切换
    X
    亮暗模式切换
    X

    在线
    客服

    在线客服
    服务时间:08:30 - 17:30

    点击下列客服在线咨询:

    服务热线

    服务
    热线

    客服服务热线

    027-67845390
    客服服务热线

    扫码

    扫码
    咨询

    微信二维码 微信扫一扫立即咨询
    返回顶部 顶部