Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Western blot analysis of MAOB using anti-MAOB antibody (BA4989). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Mouse liver tissue lysates,
Lane 2: Mouse lung tissue lysates,
Lane 3: Rat kidney tissue lysates,
Lane 4: Rat brain tissue lysates,
Lane 5: Rat liver tissue lysates,
Lane 6: Rat lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MAOB antigen affinity purified polyclonal antibody (BA4989) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MAOB at approximately 59 kDa. The expected band size for MAOB is at 59 kDa.
IHC analysis of MAOB using anti-MAOB antibody (BA4989).
MAOB was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MAOB Antibody (BA4989) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of MAOB using anti-MAOB antibody (BA4989). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Mouse liver tissue lysates,
Lane 2: Mouse lung tissue lysates,
Lane 3: Rat kidney tissue lysates,
Lane 4: Rat brain tissue lysates,
Lane 5: Rat liver tissue lysates,
Lane 6: Rat lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-MAOB antigen affinity purified polyclonal antibody (BA4989) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for MAOB at approximately 59 kDa. The expected band size for MAOB is at 59 kDa.
IHC analysis of MAOB using anti-MAOB antibody (BA4989).
MAOB was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-MAOB Antibody (BA4989) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.