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Anti-Alpha Tubulin/TUBA1B Antibody

Mouse Monoclonal Antibody

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筛选器: All WB IHC

BM1452

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 现货
  • Western blot analysis of anti- alpha Tubulin antibody (BM1452). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
    Lane 1: SH-SY5Y whole cell lysates,
    Lane 2: Jurkat whole cell lysates,
    Lane 3: A549 whole cell lysates,
    Lane 4: rat brain tissue lysates,
    Lane 5: mouse brain tissue lysates,
    Lane 6: mouse thymus tissue lysates.
    Use mouse anti- alpha Tubulin 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for alpha Tubulin at approximately 55KD. The expected band size for alpha Tubulin is at 50KD.

    all(2)
  • IHC analysis using anti- alpha Tubulin antibody (BM1452). detected in paraffin-embedded section of Human Mammary Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

    all(2)

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产品名称
Anti-Alpha Tubulin/TUBA1B Antibody
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain; TUBA1B; K-ALPHA-1
产品类型
Monoclonal
检验物种
human, mouse, rat, chicken
应用范围
WB, IHC
基因名称
Tuba1b
克隆号
DM1A
宿主
Mouse
抗体亚型
IgG1
免疫原
Microtubules from chicken embryo brain.
计算分子量
50 kDa
实际分子量
55 kDa
成分
200ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA and 50% glycerol.
纯化方式
Ascites
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Alpha-tubulin(b-alpha-1) mRNA is expressed only in brain with a molecular weight of about 55,000. The 3-prime UTR of b-alpha-1 is more than 80% homologous to the UTR of the rat brain alpha-tubulin gene, IL-alpha-T1. B-alpha-1 encodes a predicted 451-amino acid protein that is 100% identical to the rat homolog and differs by only 2 and 3 amino acids from the pig and chicken homologs, respectively.
Uniprot ID
Q71U36  
文献引用格式
Alpha Tubulin/TUBA1B Antibody (Boster Biological Technology, Wuhan, China. Catalog#BM1452)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC-免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐稀释比
Western blot (WB):1:500-2000
Immunohistochemistry (IHC):1:50-400
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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    Anti-Alpha Tubulin/TUBA1B Antibody

    筛选器: All WB IHC

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