Anti-GPX1 Antibody (Clone#IHO-7)

Rabbit monoclonal antibody

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筛选器： All WB IHC IP

BM4854

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of anti-GPX1 antibody (BM4854). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GPX1 antigen affinity purified monoclonal antibody (BM4854) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GPX1 at approximately 22 kDa. The expected band size for GPX1 is at 22 kDa.
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IHC analysis of GPX1 using anti-GPX1 antibody (BM4854) .
GPX1 was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. The tissue section was incubated with rabbit anti-GPX1 Antibody (BM4854) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of GPX1 using anti-GPX1 antibody (BM4854) .
GPX1 was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-GPX1 Antibody (BM4854) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
IHC analysis of GPX1 using anti-GPX1 antibody (BM4854) .
GPX1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-GPX1 Antibody (BM4854) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(5)
all(5)

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产品简介>

产品名称

Anti-GPX1 Antibody (Clone#IHO-7)

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

glutathione peroxidase 1; GPx 1; GPX1; GSHPx 1; GSHPX1

产品类型

Monoclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, IP

基因名称

GPX1

克隆号

IHO-7

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthesized peptide derived from human GPX1

计算分子量

22 kDa

实际分子量

22 kDa

成分

500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol.

纯化方式

Affinity-chromatography

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Glutathione peroxidase 1, also known as, GPX-1 is an enzyme that in humans is encoded by the GPX1 gene. It is mapped to 3p21.31. This gene encodes a member of the glutathione peroxidase family, consisting of eight known glutathione peroxidases (Gpx1-8) in humans. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. It has been reported that the protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell growth and increases resistance to toxic challenges. GPX1 is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA.

Uniprot ID

P07203

文献引用格式

GPX1 Antibody (Clone#IHO-7) (Boster Biological Technology, Wuhan, China. Catalog#BM4854)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Glutathione peroxidase 1

实验方案>

实验步骤

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