产品名称
Human FGF2 ELISA Kit
指标别名
Basic fibroblast growth factor; BFGF; FGF2; FGF-2; FGF 2; FGFB; HBGF 2
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
基因名全称
fibroblast growth factor 2
文献引用格式
Human FGF2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0342)
背景资料
FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96% and 97% amino acid sequence homology with mouse and rat respectively. FGF2 belongs to the fibroblast growth factor (FGF) family. Fibroblast growth factors (FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.
试剂盒内容
Catalog number | Description | Quantity |
---|
EK0342-CAP | 96-well plate precoated with anti- human FGF2 antibody | 1 |
EK0342-ST | Lyophilized recombinant human FGF2 standard | 10ng/tubex2 |
EK0342-DA | Biotinylated anti- human FGF2 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 150 | 752 | 1076 | 135 | 781 | 1071 |
Standard deviation | 10.95 | 54.89 | 79.62 | 10.8 | 62.48 | 92.1 |
CV(%) | 7.3 | 7.3 | 7.4 | 8 | 8 | 8.6 |
典型数据示例
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.123 | 0.148 | 0.191 | 0.344 | 0.576 | 0.798 | 1.572 | 2.202 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)