指标别名
BB2; CD54; ICAM 1; ICAM1; ICAM-1; P3.58; Ly 47; MALA 2
免疫原
Expression system for standard: NS0; Immunogen sequence: Q28-T493
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
intercellular adhesion molecule 1
文献引用格式
Rat ICAM1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0372)
背景资料
Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses. The standard product used in this kit is produced by recombinant gene expression, which is a dimeric protein linked with disulfide bonds.
试剂盒内容
| Catalog number | Description | Quantity |
| EK0372-CAP | 96-well plate precoated with anti- rat ICAM-1 antibody | 1 |
| EK0372-ST | Lyophilized recombinant rat ICAM-1 standard | 10ng/tube×2 |
| EK0372-DA | Biotinylated anti- rat ICAM-1 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 51 | 231 | 787 | 51 | 242 | 855 |
| Standard deviation | 3.67 | 14.55 | 42.49 | 4.23 | 16.21 | 51.3 |
| CV(%) | 7.2% | 6.3% | 5.4% | 8.3% | 6.7% | 6% |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.005 | 0.045 | 0.088 | 0.152 | 0.269 | 0.501 | 0.962 | 1.815 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
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