产品名称
Mouse IL17/IL17A ELISA Kit
指标别名
CTLA 8; CTLA8; IL 17; IL 17A; IL17; IL-17; IL17A; IL-17A; interleukin 17A
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse IL-17/IL17A ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0431)
背景资料
IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57% identical to the 17- to 26-kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri(HVS-13). IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor,and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant mouse IL-17, consisting of 137 amino acids with the molecular mass of 15.5KDa.
试剂盒内容
| Catalog number | Description | Quantity |
| EK0431-CAP | 96-well plate precoated with anti- mouse IL-17 antibody | 1 |
| EK0431-ST | Lyophilized recombinant mouse IL-17 standard | 10ng/tubex2 |
| EK0431-DA | Biotinylated anti- mouse IL-17 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 38 | 181 | 523 | 37 | 176 | 485 |
| Standard deviation | 2.35 | 10.13 | 40.27 | 2.47 | 10.91 | 41.71 |
| CV(%) | 6.2% | 5.6% | 7.7% | 6.7% | 6.2% | 8.6% |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.046 | 0.116 | 0.168 | 0.275 | 0.483 | 0.818 | 1.503 | 2.232 |
样本类型
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).