产品名称
Mouse IL18 ELISA Kit
指标别名
Iboctadekin; IFN gamma inducing factor; IGIF; IL 1 gamma; IL 18; IL 1g; IL18; IL-18; IL1F4; Interleukin 1 gamma; Interleukin 18; Mature IL18; pre IL18; Pre-IL18
免疫原
Expression system for standard: E.coli; Immunogen sequence: N36-S192
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse IL18 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0433)
背景资料
Interleukin(IL)-18, also called Interferon-gamma-inducing factor(IGIF), augments natural killer(NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1(Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0433-CAP | 96-well plate precoated with anti- mouse IL-18 antibody | 1 |
| EK0433-ST | Lyophilized recombinant mouse IL-18 standard | 10ng/tubex2 |
| EK0433-DA | Biotinylated anti- mouse IL-18 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 51 | 286 | 905 | 59 | 310 | 933 |
| Standard deviation | 2.14 | 11.15 | 41.63 | 3.07 | 17.05 | 45.72 |
| CV(%) | 4.2% | 3.9% | 4.6% | 5.2% | 5.5% | 4.9% |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.023 | 0.1 | 0.166 | 0.267 | 0.467 | 0.812 | 1.348 | 2.138 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin, EDTA)