产品名称
Mouse MIP-3 beta/CCL19 ELISA Kit
指标别名
Beta chemokine exodus 3; C C motif chemokine 19; CCL19; CK beta 11; CKb11; EBI1 ligand chemokine; ELC; MIP 3 beta; MIP 3b; MIP-3 Beta; MIP3B; MIP-3-beta; SCYA19; Small inducible cytokine A19
免疫原
Expression system for standard: E.coli; Immunogen sequence: G26-S108
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
C-C motif chemokine 19
文献引用格式
Mouse MIP-3 beta/CCL19 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0457)
背景资料
Chemokine (C-C motif) ligand 19 (CCL19) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta (MIP-3-beta). Using PCR of human-rodent hybrids, the CCL19 gene was mapped to human chromosome 9p13, and the CCL19 gene is not part of the CC chemokine gene cluster on chromosome 17. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0457-CAP | 96-well plate precoated with anti- mouse CCL19 antibody | 1 |
| EK0457-ST | Lyophilized recombinant mouse CCL19 standard | 10ng/tube×2 |
| EK0457-DA | Biotinylated anti- mouse CCL19 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 30 | 161 | 384 | 27 | 175 | 349 |
| Standard deviation | 1.2 | 8.53 | 29.56 | 1.45 | 9.27 | 33.5 |
| CV(%) | 4% | 5.3% | 7.7% | 5.4% | 5.3% | 9.6% |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.005 | 0.051 | 0.109 | 0.198 | 0.418 | 0.787 | 1.297 | 1.909 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)