产品名称
Mouse SCF/KITLG ELISA Kit
指标别名
c Kit ligand; KIT ligand; Kitl; KITLG; KL 1; Mast cell growth factor; MGF; SCF; SF; SHEP7; Stem cell factor
免疫原
Expression system for standard: E.coli; Immunogen sequence: K26-A190
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
文献引用格式
Mouse SCF/KITLG ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0509)
背景资料
Stem Cell Factor (also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that binds to the c-Kit receptor (CD117). It is mapped to 12q21.32. SCF was primarily expressed by perivascular cells throughout the bone marrow, and it plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF can exist both as a transmembrane protein and a soluble protein. This gene plays an important role in the hematopoiesis during embryonic development. During development, the presence of the SCF also plays an important role in the localization of melanocytes, cells that produce melanin and control pigmentation. In addition to it, SCF can promote mast cell adhesion, migration, proliferation, and survival. It also promotes the release of histamine and tryptase, which are involved in the allergic response.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0509-CAP | 96-well plate precoated with anti- mouse SCF antibody | 1 |
| EK0509-ST | Lyophilized recombinant mouse SCF standard | 10ng/tubex2 |
| EK0509-DA | Biotinylated anti- mouse SCF antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 75 | 190 | 820 | 81 | 186 | 861 |
| Standard deviation | 5.25 | 10.26 | 42.64 | 6.96 | 12.46 | 45.63 |
| CV(%) | 7% | 5.4% | 5.2% | 8.6% | 6.7% | 5.3% |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.05 | 0.122 | 0.178 | 0.265 | 0.431 | 0.733 | 1.275 | 1.952 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (EDTA)