Human TRAIL/TNFSF10 ELISA Kit

96T/2480

Apo 2 ligand; Apo 2L; APO2L; CD253; Protein TRAIL; TL2; TNFSF10; TRAIL

NSO,T95-G281

There is no detectable cross-reactivity with other relevant proteins.

15.6 pg/ml - 1000 pg/ml

TNFSF10

tumor necrosis factor (ligand) superfamily, member 10

P50591  

Human TRAIL/TNFSF10 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0532)

In the field of cell biology, TNF-related apoptosis-inducing ligand(TRAIL), is a protein which functions as a ligand which induces the process of cell death called apoptosis. TRAIL has also been designated CD253(cluster of differentiation 253).Tumor necrosis factor(TNF) family cytokines function as prominent mediators of immune regulation and the inflammatory response. Most TNF family cytokines are expressed as type II transmembrane proteins, with homology confined to approximately 150 C-terminal residues. The TNF ligands interact with a parallel family of receptors. TRAIL binds to the death receptors DR4(TRAIL-RI) and DR5(TRAIL-RII). The process of apoptosis is caspase-8-dependent. Caspase-8 activates downstream effector caspases including procaspase-3, -6, and -7, leading to activation of specific kinases. TRAIL also binds the receptors DcR1 and DcR2, which do not contain a cytoplasmic domain(DcR1) or contain a truncated death domain(DcR2). The standard product used in this kit is recombinant human TRAIL with the molecular mass of 21KDa. It is expressed from the amino acids ectoderm T95--G28.

15-20分钟

Catalog number	Description	Quantity
EK0532-CAP	96-well plate precoated with anti- human TRAIL antibody	1
EK0532-ST	Lyophilized recombinant human TRAIL standard	10ng/tubex2
EK0532-DA	Biotinylated anti- human TRAIL antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	38	184	416	34	175	377
Standard deviation	2.66	9.56	18.72	2.75	9.62	18.85
CV(%)	7	5.2	4.5	8.1	5.5	5

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.066	0.123	0.166	0.303	0.509	0.824	1.549	2.458

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum, Plasma (heparin, EDTA) and Saliva

3.5h