产品名称
Human E-cadherin/CDH1 ELISA Kit
指标别名
Arc 1; Cadherin 1; CD324; CDH1; CDHE; E cadherin; ECAD; E-cad; E-cadherin; LCAM; UVO; Uvomorulin
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
cadherin 1, type 1, E-cadherin (epithelial)
文献引用格式
Human E-cadherin/CDH1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0561)
背景资料
E-Cadherin, also called Cadherin 1(CDH1), Uvomorulin or calcium-dependent ashesion protein, epithelial. E-cadherin is a Ca(2+)-dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. E-cadherin plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems. The standard product used in this kit is recombinant gene expression with the molecular mass of 120KDa.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0561-CAP | 96-well plate precoated with anti- human E-Cadherin antibody | 1 |
| EK0561-ST | Lyophilized recombinant human E-Cadherin standard | 10ng/tubex2 |
| EK0561-DA | Biotinylated anti- human E-Cadherin antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 265 | 1264 | 4789 | 253 | 1258 | 4691 |
| Standard deviation | 15.37 | 58.14 | 325.65 | 16.69 | 61.64 | 318.98 |
| CV(%) | 5.8 | 4.6 | 6.8 | 6.6 | 4.9 | 6.8 |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.048 | 0.086 | 0.122 | 0.192 | 0.325 | 0.547 | 1.043 | 1.884 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin), Saliva and Urine