产品名称
Human CD30/TNFRSF8 ELISA Kit
指标别名
CD30; CD30L receptor; D1S166E; Ki 1; Ki 1 antigen; TNFRSF8
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
tumor necrosis factor receptor superfamily, member 8
文献引用格式
Human CD30/TNFRSF8 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0569)
背景资料
CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0569-CAP | 96-well plate precoated with anti- human CD30 antibody | 1 |
| EK0569-ST | Lyophilized recombinant human CD30 standard | 10ng/tube×2 |
| EK0569-DA | Biotinylated anti- human CD30 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 321 | 1322 | 3947 | 305 | 1371 | 3830 |
| Standard deviation | 25.03 | 64.77 | 157.88 | 28.06 | 93.22 | 214.48 |
| CV(%) | 7.8 | 4.9 | 4 | 9.2 | 6.8 | 5.6 |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.036 | 0.086 | 0.12 | 0.202 | 0.33 | 0.552 | 1.035 | 1.955 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)