产品名称
Mouse TNF Beta/LTA ELISA Kit
指标别名
LT; LT alpha; LTA; Lymphotoxin alpha; TNF beta; TNFB; TNF-beta; TNFSF1
免疫原
Expression system for standard: E.coli; Immunogen sequence: L34-L202
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4,000 pg/ml
文献引用格式
Mouse TNF Beta/LTA ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0585)
背景资料
The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. Alternatively spliced transcript variants have been observed for this gene.
试剂盒内容
Catalog number | Description | Quantity |
EK0585-CAP | 96-well plate precoated with anti- Mouse TNF Beta/LTA ELISA Kit A antibody | 1 |
EK0585-ST | Lyophilized recombinant Mouse TNF Beta/LTA ELISA Kit A standard | 10 ng/tube×2 |
EK0585 -DA | Biotinylated anti- Mouse TNF Beta/LTA ELISA Kit A antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 91 | 379 | 1647 | 85 | 460 | 1810 |
Standard deviation | 1.83/td> | 22.10 | 98.33 | 2.48 | 29.30 | 110.05 |
CV (%) | 2.0% | 5.8%% | 6.0% | 2.9% | 6.4% | 6.1% |
典型数据示例
Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.035 | 0.081 | 0.140 | 0.283 | 0.525 | 1.036 | 1.468 | 2.011 |
保存条件与有效期
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
cell culture supernatants, serum and plasma (heparin, EDTA or citrate).