产品名称
Mouse CTACK/CCL27 ELISA Kit
指标别名
ALP; C C motif chemokine 27; CC chemokine ILC; CCL27; CTACK; CTAK; ESKINE; IL 11 R alpha locus chemokine; ILC; PESKY; SCYA27; Skinkine; Small inducible cytokine A27
免疫原
Expression system for standard: E.coli; Immunogen sequence: L26-N120
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
C-C motif chemokine 27
文献引用格式
Mouse CTACK/CCL27 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0689)
背景资料
Chemokine (C-C motif) ligand 27 (CCL27) is a small cytokine belonging to the CC chemokine family, it is also known under the names IL-11 R-alpha-locus chemokine (ILC), Skinkine, ESkine and Cutaneous T-cell-attracting chemokine (CTACK). The human gene is mapped to 9p13.3. CCL27 is associated with homing of memory T lymphocytes to the skin, and plays a role in T cell-mediatedinflammation of the skin. And CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin. It elicits its chemotacticeffects by binding to the chemokine receptor CCR10.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0689-CAP | 96-well plate precoated with anti- mouse CCL27 antibody | 1 |
| EK0689-ST | Lyophilized recombinant mouse CCL27 standard | 10ng/tube×2 |
| EK0689-DA | Biotinylated anti- mouse CCL27 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 180 | 414 | 1713 | 188 | 393 | 1657 |
| Standard deviation | 9.18 | 24.01 | 99.35 | 11.84 | 29.47 | 107.7 |
| CV(%) | 5.1% | 5.8% | 5.8% | 6.3% | 7.5% | 6.5% |
典型数据示例
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.068 | 0.154 | 0.217 | 0.343 | 0.575 | 1.063 | 1.696 | 2.048 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)