产品名称
Mouse CCL28 ELISA Kit
指标别名
C C motif chemokine 28; CCK1; CCL28; MEC; Protein CCK1; SCYA28; Small inducible cytokine A28
免疫原
Expression system for standard: E.coli; Immunogen sequence: I23-R130
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
C-C motif chemokine 28
文献引用格式
Mouse CCL28 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0691)
背景资料
CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. It is mapped to 5p12. The tumor hypoxia promotes the recruitment of regulatory T (Treg) cells through induction of expression of the chemokine CCL28, which in turn promotes tumor tolerance and angiogenesis. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0691-CAP | 96-well plate precoated with anti- mouse CCL28 antibody | 1 |
| EK0691-ST | Lyophilized recombinant mouse CCL28 standard | 10ng/tubex2 |
| EK0691-DA | Biotinylated anti- mouse CCL28 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 79 | 209 | 835 | 80 | 199 | 814 |
| Standard deviation | 3.39 | 11.07 | 61.79 | 4.88 | 12.93 | 66.74 |
| CV(%) | 4.3% | 5.3% | 7.4% | 6.1% | 6.5% | 8.2% |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.034 | 0.123 | 0.207 | 0.337 | 0.592 | 1.019 | 1.622 | 2.386 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)