产品名称
Human CD163 ELISA Kit
指标别名
CD163; CD163 molecule; Hemoglobin scavenger receptor; M130; MM130; sCD163
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
1.56 ng/ml - 100 ng/ml
文献引用格式
Human CD163 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0715)
背景资料
CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0715-CAP | 96-well plate precoated with anti- human CD163 antibody | 1 |
| EK0715-ST0 | Lyophilized recombinant human CD163 standard | 100ng/tube×2 |
| EK0715-DA | Biotinylated anti- human CD163 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 3520 | 11456 | 44674 | 3368 | 11294 | 48653 |
| Standard deviation | 144.32 | 526.97 | 3037.83 | 195.34 | 632.46 | 3357.05 |
| CV(%) | 4.1 | 4.6 | 6.8 | 5.8 | 5.6 | 6.9 |
典型数据示例
| Concentration (ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.019 | 0.114 | 0.168 | 0.288 | 0.527 | 0.861 | 1.386 | 2.083 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Plasma (heparin)