产品名称
Human Emmprin/CD147/BSG ELISA Kit
指标别名
5F7; Basigin; basigin (Ok blood group); BSG; CD147; Collagenase stimulatory factor; EMMPRIN; HAb18G; M6; OK; OK blood group antigen; TCSF
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名全称
basigin (Ok blood group)
文献引用格式
Human Emmprin/CD147/BSG ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0751)
背景资料
Basigin is a member of the immunoglobulin superfamily that is also known as EMMPRIN, short for extracellular matrix metalloproteinase inducer, and recently has been designated CD147(cluster of differentiation 147). It is a member of the immunoglobulin superfamily, with a structure related to the putative primordial form of the family. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, basigin is thought also to play a role in intercellular recognition(Miyauchi et al., 1991; Kanekura et al., 1991).This protein is a determinant for the Ok blood group system. Basigin is a type I integral membrane receptor that has many ligands, including the cyclophilin(CyP) proteins Cyp-A and CyP-B and certain integrins. It is expressed by many cell types, including epithelial cells, endothelial cells and leukocytes.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0751-CAP | 96-well plate precoated with anti- human Emmprin antibody | 1 |
| EK0751-ST | Lyophilized recombinant human Emmprin standard | 10ng/tube×2 |
| EK0751-DA | Biotinylated anti- human Emmprin antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 69 | 232 | 1065 | 70 | 210 | 975 |
| Standard deviation | 5.24 | 16.24 | 76.68 | 5.32 | 15.54 | 77.02 |
| CV(%) | 7.6 | 7 | 7.2 | 7.6 | 7.4 | 7.9 |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.028 | 0.102 | 0.152 | 0.257 | 0.429 | 0.735 | 1.234 | 2.018 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA), Saliva, Urine and Human Milk
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