产品名称
Mouse Endocan/ESM1 ELISA Kit
指标别名
Endothelial cell-specific molecule 1; ESM-1; ESM1; Endocan
免疫原
Expression system for standard: NS0; Immunogen sequence: W20-R184
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Endothelial cell-specific molecule 1
文献引用格式
Mouse Endocan/ESM1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0753)
背景资料
Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM-1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF (vascular endothelial growth factor) or FGF-2 (fibroblast growth factor 2).
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0753-CAP | 96-well plate precoated with anti- mouse ESM1 antibody | 1 |
| EK0753-ST | Lyophilized recombinant mouse ESM1 standard | 10ng/tube×2 |
| EK0753-DA | Biotinylated anti- mouse ESM1 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 56 | 169 | 683 | 60 | 163 | 636 |
| Standard deviation | 3.36 | 10.14 | 52.59 | 4.2 | 12.22 | 56.6 |
| CV(%) | 6% | 6% | 7.7% | 7% | 7.5% | 8.9% |
典型数据示例
| Concentration (pg/ml) | 0 | 23.4 | 46.8 | 93.7 | 187.5 | 375 | 750 | 1500 |
| O.D. | 0.071 | 0.124 | 0.179 | 0.283 | 0.498 | 0.88 | 1.609 | 2.399 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)