产品名称
Mouse HAI-2/SPINT2 ELISA Kit
指标别名
DIAR3; FLJ45571; HAI-2; HAI2; Kop; PB
免疫原
Expression system for standard: NS0; Immunogen sequence: R30-K140
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Kunitz-type protease inhibitor 2
文献引用格式
Mouse HAI-2/SPINT2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0774)
背景资料
Kunitz-type protease inhibitor 2 is an enzyme inhibitor that in humans is encoded by the SPINT2 gene. This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. And this gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0774-CAP | 96-well plate precoated with anti- mouse HAI-2 antibody | 1 |
| EK0774-ST | Lyophilized recombinant mouse HAI-2 standard | 10ng/tube×2 |
| EK0774-DA | Biotinylated anti- mouse HAI-2 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 61 | 240 | 837 | 64 | 248 | 772 |
| Standard deviation | 4.14 | 13.92 | 46.03 | 5.56 | 17.85 | 55.58 |
| CV(%) | 6.8% | 5.8% | 5.5% | 8.7% | 7.2% | 7.2% |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.036 | 0.172 | 0.245 | 0.391 | 0.618 | 1.017 | 1.57 | 2.395 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Cell Lysates