产品名称
Human IL17E/IL25 ELISA Kit
指标别名
interleukin 25; Interleukin-17E; IL17E; IL25; IL-25; IL 25; UNQ3120/PRO10272
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
62.5 pg/ml - 4000 pg/ml
文献引用格式
Human IL-17E/IL25 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0793)
背景资料
Interleukin-25 (IL-25), also known as interleukin-17E (IL-17E), is a protein that in humans is encoded by the IL25 gene. It is a cytokine that belongs to the IL-17 cytokine family and mapped to 14q11.2. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. It has been found that IL-25 can induce Th2-type cytokine expression and production by non-T, non-B accessory cells that express high levels of major histocompatibility complex class II and low levels of Cd11c. This cytokine also can induce NF-κB activation, and stimulate the production of IL8. What’s more, IL-25 is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0793-CAP | 96-well plate precoated with anti- human IL-17E antibody | 1 |
| EK0793-ST | Lyophilized recombinant human IL-17E standard | 10ng/tube×2 |
| EK0793-DA | Biotinylated anti- human IL-17E antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 162 | 689 | 1026 | 168 | 748 | 1001 |
| Standard deviation | 8.91 | 42.02 | 50.27 | 10.92 | 57.59 | 53.05 |
| CV(%) | 5.5 | 6.1 | 4.9 | 6.5 | 7.7 | 5.3 |
典型数据示例
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.05 | 0.086 | 0.143 | 0.269 | 0.504 | 0.925 | 1.614 | 2.102 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)