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Human P53/TP53 ELISA Kit

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EK0895

  • 96T ¥2480
  • 货期: 现货

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产品名称
Human P53/TP53 ELISA Kit
规格/价格
96T/2480
指标别名
Antigen NY CO 13; Cellular tumor antigen p53; LFS1; P53; Phosphoprotein p53; TP53; TRP53; tumor protein p53; Tumor suppressor p53
免疫原
NSO, M1-D393
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名称
TP53
基因名全称
tumor protein p53
Uniprot ID
P04637  
文献引用格式
Human P53/TP53 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0895)
背景资料
P53(also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. The human P53 gene is mapped to chromosome 17. Human p53 is 393 amino acids long and has seven domains. It runs as a 53-kilodalton(kDa) protein on SDS-PAGE. The pattern of p53 splicing was specific for brain areas and for individuals. And human kidney and heart expressed only full-length p53. It has played a vital role in conserving stability by preventing genome mutation.
显色时间
25-30分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0895-CAP96-well plate precoated with anti- human P53 antibody1
EK0895-STLyophilized recombinant human P53 standard10ng/tube×2
EK0895-DABiotinylated anti- human P53 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)2751540505530213965437
Standard deviation16.572.38394.2920.8371.19467.58
CV(%)64.77.86.95.18.6
典型数据示例
Concentration(pg/ml)015631262512502500500010000
O.D.0.0280.0710.1200.2250.3650.6641.2832.001
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
应用范围
ELISA
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Lysates
实验时长
3.5h

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