Catalog number | Description | Quantity |
---|---|---|
EK0895-CAP | 96-well plate precoated with anti- human P53 antibody | 1 |
EK0895-ST | Lyophilized recombinant human P53 standard | 10ng/tube×2 |
EK0895-DA | Biotinylated anti- human P53 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 275 | 1540 | 5055 | 302 | 1396 | 5437 |
Standard deviation | 16.5 | 72.38 | 394.29 | 20.83 | 71.19 | 467.58 |
CV(%) | 6 | 4.7 | 7.8 | 6.9 | 5.1 | 8.6 |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.028 | 0.071 | 0.120 | 0.225 | 0.365 | 0.664 | 1.283 | 2.001 |
Human P53 ELISA Kit standard curve
Human P53 ELISA Kit standard curve