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Human PEDF/SERPINF1 ELISA Kit

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EK0896

  • 96T ¥2480
  • 货期: 现货
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产品名称
Human PEDF/SERPINF1 ELISA Kit
规格/价格
96T|2480
指标别名
EPC 1; PEDF; PIG35; Serpin F1; SERPINF1
免疫原
NSO, M1-P418
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156pg/ml-10000pg/ml
基因名称
SERPINF1
基因名全称
serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
Uniprot ID
P36955  
文献引用格式
Human PEDF/SERPINF1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0896)
背景资料
Pigment epithelium-derived factor (PEDF) is also known as serpin F1 (SERPINF1). In humans, it is encoded by the SERPINF1 gene. PEDF has a variety of functions including antiangiogenic, antitumorigenic, and neurotrophic properties. It suppresses retinal neovascularization and endothelial cell proliferation. And Antiangiogenic function is also conferred by PEDF through inhibition of both VEGFR-1 and VEGFR-2. In addition, the antitumorigenic effects of PEDF are not only due to inhibition of supporting vasculature, but also due to effects on the cancer cells themselves. PEDF is shown to inhibit cancer cell proliferation and increase apoptosis via the FAS/FASL pathway. Expression of PEDF in the human retina is found at 7.4 weeks of gestation, suggesting it may play a role in retinal neuron differentiation.
显色时间
25-30分钟
试剂盒内容
Catalog numberDescriptionQuantity
EK0896-CAP96-well plate precoated with anti- human PEDF antibody1
EK0896-STLyophilized recombinant human PEDF standard10ng/tube×2
EK0896-DABiotinylated anti- human PEDF antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30 ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)11355918205091124543918927
Standard deviation76.04337.32820.3680.92364.41908.49
CV(%)6.75.747.26.74.8
典型数据示例
Concentration (pg/ml)015631262512502500500010000
O.D.0.0060.0610.1170.2140.4050.7221.2971.834
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
检验物种
human
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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