产品名称
Human PEDF/SERPINF1 ELISA Kit
指标别名
EPC 1; PEDF; PIG35; Serpin F1; SERPINF1
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
文献引用格式
Human PEDF/SERPINF1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0896)
背景资料
Pigment epithelium-derived factor (PEDF) is also known as serpin F1 (SERPINF1). In humans, it is encoded by the SERPINF1 gene. PEDF has a variety of functions including antiangiogenic, antitumorigenic, and neurotrophic properties. It suppresses retinal neovascularization and endothelial cell proliferation. And Antiangiogenic function is also conferred by PEDF through inhibition of both VEGFR-1 and VEGFR-2. In addition, the antitumorigenic effects of PEDF are not only due to inhibition of supporting vasculature, but also due to effects on the cancer cells themselves. PEDF is shown to inhibit cancer cell proliferation and increase apoptosis via the FAS/FASL pathway. Expression of PEDF in the human retina is found at 7.4 weeks of gestation, suggesting it may play a role in retinal neuron differentiation.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0896-CAP | 96-well plate precoated with anti- human PEDF antibody | 1 |
| EK0896-ST | Lyophilized recombinant human PEDF standard | 10ng/tube×2 |
| EK0896-DA | Biotinylated anti- human PEDF antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1135 | 5918 | 20509 | 1124 | 5439 | 18927 |
| Standard deviation | 76.04 | 337.32 | 820.36 | 80.92 | 364.41 | 908.49 |
| CV(%) | 6.7 | 5.7 | 4 | 7.2 | 6.7 | 4.8 |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.006 | 0.061 | 0.117 | 0.214 | 0.405 | 0.722 | 1.297 | 1.834 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)