产品名称
Human Aggrecan/ACAN ELISA Kit
指标别名
ACAN; AGC1; AGCAN; aggrecan; CSPG1; CSPGCP; MSK16; SEDK
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
1.56 ng/ml - 100 ng/ml
文献引用格式
Human Aggrecan/ACAN ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0909)
背景资料
Aggrecan, also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. It is a critical component for cartilage structure and the function of joints. Aggrecan provides intervertebral disc and cartilage with the ability to resist compressive loads. The localized high concentrations of aggrecan provide the osmotic properties necessary for normal tissue function with the GAGs producing the swelling pressure that counters compressive loads on the tissue. This functional ability is dependent on a high GAG/aggrecan concentration being present in the tissue extracellular matrix. Also, Aggrecan plays an important role in the organization of the extracellular spaces between neurons in the brain.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0909-CAP | 96-well plate precoated with anti- human Aggrecan antibody | 1 |
| EK0909-ST0 | Lyophilized recombinant human Aggrecan standard | 100ng/tube×2 |
| EK0909-DA | Biotinylated anti- human Aggrecan antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 3084 | 13064 | 43102 | 3322 | 13982 | 40208 |
| Standard deviation | 240.55 | 679.32 | 1939.59 | 275.72 | 908.83 | 1809.36 |
| CV(%) | 7.8 | 5.2 | 4.5 | 8.3 | 6.5 | 4.5 |
典型数据示例
| Concentration (ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.047 | 0.116 | 0.147 | 0.226 | 0.422 | 0.709 | 1.212 | 1.904 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum and Urine