产品名称
Human CD23/FCER2 ELISA Kit
指标别名
BLAST 2; CD23; CD23A; CLEC4J; Fc epsilon RII; FCE2; FCER2; IGEBF; Lymphocyte IgE receptor
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
31.2 pg/ml - 2000 pg/ml
基因名全称
Fc fragment of IgE, low affinity II, receptor for (CD23)
文献引用格式
Human CD23/FCER2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0912)
背景资料
CD23, also known as Fc epsilon RII, or FcεRII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the FCE2 gene was assigned to 19p13.3. CD23(FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0912-CAP | 96-well plate precoated with anti- human CD23 antibody | 1 |
| EK0912-ST | Lyophilized recombinant human CD23 standard | 10ng/tube×2 |
| EK0912-DA | Biotinylated anti- human CD23 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 61 | 194 | 981 | 55 | 207 | 899 |
| Standard deviation | 2.8 | 7.95 | 64.74 | 3.41 | 9.93 | 71.02 |
| CV(%) | 4.6 | 4.1 | 6.6 | 6.2 | 4.8 | 7.9 |
典型数据示例
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.002 | 0.056 | 0.108 | 0.199 | 0.356 | 0.608 | 1.155 | 2.001 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine