产品名称
Human COMP ELISA Kit
指标别名
COMP; EDM1; EPD1; MED; PSACH; THBS5; Thrombospondin 5; TSP5
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
156 pg/ml - 10000 pg/ml
基因名全称
cartilage oligomeric matrix protein
文献引用格式
Human COMP ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0913)
背景资料
Cartilage oligomeric matrix protein is a protein that in humans is encoded by the COMP gene. The sequences of rat and bovine COMP indicate that it is a member of the thrombospondin gene family. By Southern blot analysis of a somatic cell hybrid DNA panel and by isotopic in situ hybridization, human COMP gene was mapped to 19p13.1, and the murine COMP gene was mapped to the central region of mouse chromosome 8 by use of an interspecific backcross mapping panel. COMP is a marker of cartilage turnover.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0913-CAP | 96-well plate precoated with anti- human COMP antibody | 1 |
| EK0913-ST | Lyophilized recombinant human COMP standard | 10ng/tube×2 |
| EK0913-DA | Biotinylated anti- human COMP antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 233 | 1997 | 4935 | 255 | 1893 | 5413 |
| Standard deviation | 9.78 | 137.79 | 246.75 | 12.49 | 138.18 | 281.47 |
| CV(%) | 4.2 | 6.9 | 5 | 4.9 | 7.3 | 5.2 |
典型数据示例
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.02 | 0.066 | 0.12 | 0.231 | 0.462 | 0.918 | 1.816 | 2.572 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)