产品名称
Mouse YKL-40/CHI3L1 ELISA Kit
指标别名
39 kDa synovial protein; ASRT7; Cartilage glycoprotein 39; CGP 39; CHI3L1; CHI3L1/YKL40; Chitinase 3 like protein 1; Chitinase 3-like 1; GP 39; GP39; HC gp39; hCGP 39; HCGP 3P; YKL 40; YKL40; YYL 40
免疫原
Expression system for standard: NS0; Immunogen sequence: Y22-A381
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Chitinase-3-like protein 1
文献引用格式
Mouse YKL-40/CHI3L1 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0975)
背景资料
Chitinase-3-like protein 1, also called CHI3L1 or YKL-40 is a secreted glycoprotein that is approximately 40kDa in size that in humans is encoded by the CHI3L1 gene. This gene is mapped to 1q32.1. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK0975-CAP | 96-well plate precoated with anti- mouse Chitinase 3-like 1 antibody | 1 |
| EK0975-ST | Lyophilized recombinant mouse Chitinase 3-like 1 standard | 10ng/tube×2 |
| EK0975-DA | Biotinylated anti- mouse Chitinase 3-like 1 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 176 | 647 | 1183 | 170 | 667 | 1185 |
| Standard deviation | 9.68 | 32.99 | 81.62 | 12.58 | 43.35 | 103.09 |
| CV(%) | 5.5% | 5.1% | 6.9% | 7.4% | 6.5% | 8.7% |
典型数据示例
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.011 | 0.081 | 0.146 | 0.259 | 0.483 | 0.859 | 1.498 | 2.333 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)