产品名称
Human CCL15 ELISA Kit
指标别名
C-C motif chemokine ligand 15; HCC-2; HMRP-2B; LKN-1; LKN1; MIP-1 delta; MIP-1D; MIP-5; MRP-2B; NCC-3; NCC3; SCYA15; SCYL3; SY15; CCL15
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名全称
chemokine (C-C motif) ligand 15
文献引用格式
Human CCL15 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1120)
背景资料
Chemokine(C-C motif) ligand 15(CCL15) is a small cytokine belonging to the CC chemokine family that is also known as leukotactin-1, MIP5 and HCC-2. CCL15 is expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3. The human CCL15 gene spans four exons and is located in a head-to-tail orientation on chromosome 17 with the gene of another CC chemokine known as CCL14.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1120-CAP | 96-well plate precoated with anti- human CCL15 antibody | 1 |
| EK1120-ST | Lyophilized recombinant human CCL15 standard | 10ng/tube×2 |
| EK1120-DA | Biotinylated anti- human CCL15 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 33 | 185 | 439 | 31 | 188 | 417 |
| Standard deviation | 2.31 | 9.8 | 31.6 | 2.75 | 13.34 | 37.11 |
| CV(%) | 7 | 5.3 | 7.2 | 8.9 | 7.1 | 8.9 |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.027 | 0.093 | 0.129 | 0.224 | 0.421 | 0.725 | 1.326 | 2.003 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)