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Mouse MCP5/CCL12 ELISA Kit

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EK1128

  • 96T ¥2480
  • 货期: 现货
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产品名称
Mouse MCP5/CCL12 ELISA Kit
规格/价格
96T|2480
指标别名
Ccl12; MCP 5; MCP-5; Scya12
免疫原
Expression system for standard: E.coli; Immunogen sequence: G23-G104
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名称
Ccl12
基因名全称
C-C motif chemokine 12
Uniprot ID
Q62401  
文献引用格式
Mouse MCP5/CCL12 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1128)
背景资料
Chemokine (C-C motif) ligand 12 (CCL12) is a small cytokine belonging to the CC chemokine family that has been described in mice. It is also known as monocyte chemotactic protein 5 (MCP-5) and, due to its similarity with the human chemokine MCP-1, sometimes it is called MCP-1-related chemokine. CCL12 specifically attracts eosinophils, monocytes and lymphocytes. This chemokine is found predominately in lymph nodes and thymus under normal conditions, and its expression can be hugely induced in macrophages. It is thought to coordinate cell movements during early allergic reactions, and immune response to pathogens. The gene for CCL12 is found in a cluster of CC chemokines on mouse chromosome 11.
试剂盒内容
Catalog numberDescriptionQuantity
EK1128-CAP96-well plate precoated with anti- mouse CCL12 antibody1
EK1128-STLyophilized recombinant mouse CCL12 standard10ng/tube×2
EK1128-DABiotinylated anti- mouse CCL12 antibody100μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)100μl(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
批内差/批间差
Intra-Assay Precision
Inter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)30673333071324
Standard deviation0.703.0818.780.665.5915.33
CV (%)2.3%4.6%5.6%2.2%7.9%4.7%
典型数据示例
Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0420.1060.1580.2530.460.8831.532.088
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
检验物种
mouse
检测方法
酶联免疫双抗夹心法
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)
实验时长
3.5h

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