产品名称
Mouse MCP5/CCL12 ELISA Kit
指标别名
Ccl12; MCP 5; MCP-5; Scya12
免疫原
Expression system for standard: E.coli; Immunogen sequence: G23-G104
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
检测范围
15.6 pg/ml - 1000 pg/ml
基因名全称
C-C motif chemokine 12
文献引用格式
Mouse MCP5/CCL12 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1128)
背景资料
Chemokine (C-C motif) ligand 12 (CCL12) is a small cytokine belonging to the CC chemokine family that has been described in mice. It is also known as monocyte chemotactic protein 5 (MCP-5) and, due to its similarity with the human chemokine MCP-1, sometimes it is called MCP-1-related chemokine. CCL12 specifically attracts eosinophils, monocytes and lymphocytes. This chemokine is found predominately in lymph nodes and thymus under normal conditions, and its expression can be hugely induced in macrophages. It is thought to coordinate cell movements during early allergic reactions, and immune response to pathogens. The gene for CCL12 is found in a cluster of CC chemokines on mouse chromosome 11.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1128-CAP | 96-well plate precoated with anti- mouse CCL12 antibody | 1 |
| EK1128-ST | Lyophilized recombinant mouse CCL12 standard | 10ng/tube×2 |
| EK1128-DA | Biotinylated anti- mouse CCL12 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 30 | 67 | 333 | 30 | 71 | 324 |
| Standard deviation | 0.70 | 3.08 | 18.78 | 0.66 | 5.59 | 15.33 |
| CV (%) | 2.3% | 4.6% | 5.6% | 2.2% | 7.9% | 4.7% |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.042 | 0.106 | 0.158 | 0.253 | 0.46 | 0.883 | 1.53 | 2.088 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)