产品名称
Mouse GDF15 ELISA Kit
指标别名
GDF-15; GDF 15; GDF15; MIC 1; MIC1; NAG 1; NRG 1; NSAID activated gene 1 protein; NSAID regulated gene 1 protein; PDF; PLAB; Placental TGF beta; PTGFB
免疫原
Expression system for standard: NS0; Immunogen sequence: S189-A303
交叉反应
There is no detectable cross-reactivity with other relevant proteins.
基因名全称
Growth/differentiation factor 15
文献引用格式
Mouse GDF15 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK1134)
背景资料
GDF-15 (Growth differentiation factor 15),also known as TGF-PL, MIC-1, PDF, PLAB, and PTGFB, is a protein belonging to the transforming growth factor beta superfamily that has a role in regulating inflammatory and apoptotic pathways in injured tissues and during disease processes. Using FISH, the MIC1 gene is mapped to 19p13.2-p13.1. Its expression in liver can be significantly up-regulated in during injury of organs such as liver, kidney, heart and lung. GDF15 showed increased expression and secretion during erythroblast maturation. GDF15 functions as an antiinflammatory cytokine by directly interfering with chemokine signaling and integrin activation.
试剂盒内容
| Catalog number | Description | Quantity |
|---|
| EK1134-CAP | 96-well plate precoated with anti- mouse GDF-15 antibody | 1 |
| EK1134-ST | Lyophilized recombinant mouse GDF-15 standard | 1ng/tube×2 |
| EK1134-DA | Biotinylated anti- mouse GDF-15 antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision |
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 36 | 233 | 386 | 39 | 238 | 388 |
| Standard deviation | 1.76 | 10.01 | 23.16 | 2.45 | 14.28 | 25.6 |
| CV(%) | 4.9% | 4.3% | 6% | 6.3% | 6% | 6.6% |
典型数据示例
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.041 | 0.092 | 0.14 | 0.252 | 0.396 | 0.707 | 1.105 | 1.849 |
保存条件与有效期
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
样本类型
Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)